Experimental investigation on growth profiles of microalgae with different flow conditions in photo-bioreactors
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摘要: 高密度藻培对藻类资源高效利用十分重要。为解决流动条件对微藻生长作用机理不明的问题，本文借助高时间分辨率粒子图像测速技术（Time Resolved Particle Image Velocimeter, TR-PIV），对比研究光生物反应器(Photobioreactors, PBR)的水流速度分布特征和涡流效应，获得了藻液流速、涡量、湍流动能（TKE）云图，测量了小球藻的生长速率和类胡萝卜素含量。实验结果表明：高轴向速度、高径向速度、高涡量（0.015 s−1≤Ω≥0.025 s−1）、高TKE(k≤0.2 m2/s2)的流动会加速小球藻细胞的分裂、生长及高附加值产物产生；流场可视化方法是PBR设计与优化的一种有效工具。Abstract: Well-designed Photo-bioreactors (PBR) support high-efficiency biomass production. Methods: Flow fields of plat-plate PBR are experimental investigated with a time-resolved particle image velocimeter (TR-PIV). Results: Microalgae flow around sets of baffles improves performance of Chlorella vulgaris growth. Conclusion: The PIV-based flow visualization method benefits microalgae PBR designs, implements of high-density cultivations. The PBR structures proposed in this paper is of great supports to design and high productivity of bio-products with high added-values.
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Key words:
- Photo-bioreactor /
- Flow visualization /
- TR-PIV /
- Microalgae /
- High density cultivation
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图 3 PIV系统拍摄的YZ截面藻液瞬时流速
Figure 3. PIV captured velocity and streamline contours of algal liquid
图 4 PIV系统拍摄的不同曝气流量的藻液平均流速云图
Figure 4. PIV captured averaged velocity contours of algal liquid
图 5 对比PBR藻液脉动速度图
Figure 5. UY-axis and VZ-axis fluctuating velocities of algal liquids
图 6 PIV系统拍摄的PBR藻液轴向速度和径向速度
Figure 6. VZ-axis and UY-axis velocity profiles of algal liquids
图 7 PIV系统拍摄的藻液时均速度矢量云图
Figure 7. PIV captured velocity vector contours of algal liquid
图 10 PIV系统拍摄的时均湍流动能云图
Figure 10. PIV captured turbulent kinetic energy contours of algal liquid
图 12 流动影响参数a与藻生长、生物质产率的关联图
Figure 12. Relationship between a, algae growth, biomass productivity
表 1 小球藻培养结果
Table 1. Chlorella vuguris cultivation data
培养时间/天 I/A 1# PBR 2# PBR 3# PBR 0 0.179 0.179 0.179 2 0.169 0.353 0.303 4 0.202 1.124 0.962 6 0.245 2.332 2.240 7 0.225 3.220 3.036 8 0.233 4.963 4.473 10 0.342 6.083 5.684 
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