原子力显微镜的生物力学实验方法和研究进展

关东石; 李航宇; 童彭尔

doi:10.11729/syltlx20200026

原子力显微镜的生物力学实验方法和研究进展

doi: 10.11729/syltlx20200026

1.
中国科学院力学研究所非线性力学国家重点实验室, 北京 100190
2.
香港科技大学物理系, 香港

基金项目:

国家自然科学基金 11972351

详细信息

作者简介:
关东石(1987-), 男, 吉林通化人, 中国科学院力学研究所非线性力学国家重点实验室项目研究员。研究方向:微纳尺度流体力学及其交叉和应用。通信地址:北京市海淀区北四环西路15号中国科学院力学研究所非线性力学国家重点实验室(100190)。E-mail:dsguan@imech.ac.cn

通讯作者:
关东石, E-mail:dsguan@imech.ac.cn

中图分类号: O35
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- 被引次数: 0
出版历程
- 收稿日期: 2020-03-01
- 修回日期: 2020-03-23
- 刊出日期: 2020-04-25

Experimental methods and recent progress in biomechanics using atomic force microscopy

1.
The State Key Laboratory of Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China
2.
Department of Physics, The Hong Kong University of Science and Technology, Hong Kong, China

摘要

摘要: 作为微纳尺度的力学工具，原子力显微镜技术被越来越多地应用于生物力学实验研究，推动了该交叉学科领域的发展。利用多种测量模式与改进的探针，原子力显微镜可以在液体中对亚细胞、细胞、组织等多个尺度的生命物质进行力学测量，研究其在衰老、癌变等生命过程中力学性质的动态变化。本文综述了原子力显微镜的力学测量原理、生物力学的实验方法，以及在单细胞的整体与局部、液-液相分离液滴、上皮囊泡组织等力学测量中的应用，分析了复杂流体与微纳尺度流动对实验测量的影响，并对该领域的发展进行了展望。
- 原子力显微镜 /
- 生物力学 /
- 微纳流动 /
- 弹性模量 /
- 软物质
Abstract: As a micro-and nano-scale mechanical tool, Atomic Force Microscopy (AFM) is increasingly used in the experimental study of the biomechanics and promotes the further development of this interdisciplinary field. Using a variety of operation modes and modified probes, the AFM can carry out mechanical measurements on living matter at multiple scales, from subcellular structures to living cells and tissues. It can be used to study variations of the mechanical properties during different living processes, such as aging and cancerization. In this article, we will review the working principle of AFM, its experimental implementations in biomechanical measurements, and the applications of the AFM in the study of the mechanical properties of the whole cell and local variations, liquid-liquid phase-separated droplets, and epithelial cysts. We also analyze the effects of complex fluids and micro-and nano-scale flows on the AFM measurements, and make an outlook on the development of this field.
- Atomic Force Microscopy (AFM) /
- biomechanics /
- micro-and nano-fluidics /
- elastic modulus /
- soft matter

HTML全文

图 1 原子力显微镜示意图

Figure 1. A schematic of an AFM

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图 2 振幅A和相位φ在共振频率附近随驱动频率ω的变化

Figure 2. The amplitude A and phase φ as a function of driving frequency ω near resonance ω₀

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图 3 各种原子力显微镜探针^[36]

Figure 3. Different types of AFM probes^[36]

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图 4 胶体探针力压痕实验

Figure 4. The force-indentation experiment with a colloidal probe

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图 5 利用胶体探针对液-液相分离所形成的神经元突触后致密区的蛋白质液滴进行的力学测量^[35]

Figure 5. Mechanical measurement of the postsynaptic density protein droplets formed by liquid-liquid phase separation using an AFM colloidal probe^[35]

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图 6 球形上皮囊泡的荧光与明场图像以及力压痕曲线^[34]

Figure 6. The fluorescence and bright-flied images and the force-indentation curves of the epithelial cyst^[34]

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图 7 在活细胞上测量力反馈函数K^* (D) ^[36]

Figure 7. Measured response functionK^* (D)for a living cell^[36]

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图 8 原子力显微镜通过双频调制同时进行形貌扫描与黏弹性测量的控制流程图^[36]

Figure 8. A flowchart showing the AFM controls for simultaneous topographic and viscoelastic mapping with dual-frequency modulations^[36]

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图 9 原子力显微镜测得的活细胞三维形貌和弹性图像^[36]

Figure 9. AFM images of three-dimensional topography and elasticity of a living cell at interphase and mitosis^[36]

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